quantitative westerns

David Martin x3175 dmartin at crc.ac.uk
Wed May 5 02:57:02 EST 1993

The ecl signal is semi quantitative.. but you have to run a standard curve on
each western.. (and make sure you spread the ECL reagent evenly.) but then 
again most protein assays are semi quantitative, just some are more semi than 
quantitative. We have put a gradient of antigen on a blot and then
 densitometrically scanned it. the density corresponds to the antigen in
a linear sort of fashion.

Suck it and see is all I can suggest. Try a gradient of your antigen and
see what sort of results you get.

hope this helps

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