linear DNA Xformation

Tibor WEIS,network manager tibor at
Tue May 4 11:02:14 EST 1993

In article <C5r7yA.6vs at> pnh at (Paul N Hengen) writes:
>Relay-Version: VMS News - V6.1B5 17/9/92 VAX/VMS V5.4-1; site mvax.uakom.cs
>Path: mvax.uakom.cs!aci.cvut.cs!alijku11!!!!
>Newsgroups: bionet.molbio.methds-reagnts
>Subject: Re: linear DNA Xformation
>Message-ID: <C5r7yA.6vs at>
>From: pnh at (Paul N Hengen)
>Date: Mon, 19 Apr 1993 23:30:10 GMT
>Sender: Paul N. Hengen
>References: <1993Apr19.094503.26490 at>
>Distribution: bionet
>Organization: Frederick Cancer Research and Development Center
>Summary: Reference
>Lines: 40
>IBELGAUFTS at de.mpg.biochem.vms wrote:
>: Are there any reasons why transformation of bacteria with linear DNA
>: should not work? Does it work?
>Mike Poidinger wrote:
>> It depends on why you want to do it.
>> My understanding is that Eukaryotic Xformations are done
>> with linear DNA to improve the eficiency of integration into
>> the chromosome. Since most bacterial Xformations involve
>> autonomously replicating plasmids, linearizing the
>> DNA would not serve much purpose. And if you actually wanted
>> chromosomal integration, I would guess there are better ways
>> than Xformation with linear DNA and then hoping :-)
>It could be you would like to force an integration or perhaps
>cause a specific mutation within the bacterial chromosome.
>See the following article for a method to do such a thing:
>author = "S. C. Winans
>     and S. J. Elledge
>     and J. H. Krueger
>     and G. C. Walker",
>title = "Site-directed insertion and deletion mutagenesis
>with cloned fragments in Escherichia coli",
>journal = "J. Bacteriol.",
>volume = "161",
>pages = "1219-1221",
>year = "1985"}
>Paul N. Hengen
>National Cancer Institute
>Frederick Cancer Research and Development Center
>Frederick, Maryland 21702-1201 USA

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