Polka dot background

[Klaus.Matthaei at anu.edu.au]

>I have been screening phage lifts on NC filters with kinased oligos and have 
>been getting a lot of "polka dot" background. Does any one know what causes 
>this and how to avoid it. I never get this with RNA or DNA probes.
>
>    Condie E. Carmack     ====================================================
> GenPharm International   ====================================================
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Hi 

My guess would be excessively high levels of unincorporated nucleotides.

Do you check your level of incorporation??  I have a rapid method for
radiolabelled probes using a PEI tlc plate if you would like.

Cheers, Klaus
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Klaus Matthaei
Gene Targeting
The John Curtin School of Medical Research
The Australian National University
E-mail: Klaus.Matthaei at anu.edu.au

"If you don't do: you rust"
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