Use of KCl for PAGE staining

Shaun D. Black SHAUN%JASON.DECNET at relay.the.net
Thu May 6 14:24:57 EST 1993


I thought I might add one further item to Al McGraw's question on KCl as a 
reagent for SDS-PAGE staining to recover protein for use as an antigen.  
There was a recent report in BioTechniques (12:564-573 (1992)) entitled 
"Reverse Staining of Sodium Dodecy Sulfate Po;yacrylamide Gels by Imadazole
-Zinc Salts: Sensitive Detection of Unmodified Proteins".  I haven't used 
the method, but their data look quite good.  The stain uses readily 
accessible reagents, has very low background, is rapid, is as sensitive as 
a silver stain, is reversible, and can be used with Western Blotting and
Edman sequencing.  Let us know if anyone finds this useful.  Cheers.  -Shaun
  =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= 
  = Shaun D. Black, PhD     | Internet:  shaun%jason.decnet at relay.the.net = 
  = Dept. of Biochemistry   | University of Texas Health Center  at Tyler =
  =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= 



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