CAT sequencing primers

David Roos droos at sas.upenn.edu
Fri May 7 11:47:36 EST 1993


**WARNING** -- Primer 5'-AATAAGCGGATGAATGGCAG-3' mentioned in a recent posting
is located ~50 nt downstream of the stop codon, and therefore suitable for
use only with downstream inserts (and only if your CAT cassette includes this
much of the 3' non-coding region).  For an antisense primer suitable for
sequencing 5' inserts,try 5'-TGCCATTGGGATATATCAACGGTG-3', as mentioned a
couple of days ago. -- D.S.Roos




More information about the Methods mailing list