CNBr cleavage of MBP-fusion protein?

kaya at mscf.med.upenn.edu kaya at mscf.med.upenn.edu
Mon May 10 16:23:48 EST 1993


To anyone using the pMal protein fusion and purification system from NEB or 
anyone familiar with cyanogen bromide cleavages:

Are any of you using cyanogen bromide to cleave the fusion protein?

I have been told by somebody that the maltose (10mM)in the column elution 
buffer can cause incomplete cleavage in the cleavage step.  It was recommended
that I dialyze before the cleavage step.

Can anyone confirm this observation? 

I would appreciate any feedback I can get on this.  Thanks!

Angie.



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