Cell Culture Contamination

Andre Hamel hamel at ccu.umanitoba.ca
Tue May 11 09:54:40 EST 1993

Have you tried including antifungal agents in your TC media?
Furthermore .. perhaps your media itself is contaminated with fungi.

Andre Hamel                                 email: hamel at ccu.umanitoba.ca
Manitoba Veterinary Services                lab tel.: (204) 945-7630
Infectious & Genetic Disease                home tel.: (204) 275-1204
Mol.Biol.Lab                                FAX: (204) 945-8062
545 University Crescent                   
Winnipeg, Manitoba                          
R3T 5S6

In article <C6tx4E.LEv at news.cso.uiuc.edu> erwg0233 at uxa.cso.uiuc.edu (Erik Robert Wilson) writes:
>We have been getting some weird looking gunk in our cell cultures
>and I was hoping that someone has some idea what the problem was,
>what to do about it or at least what would be a good place to find
>some usefull information.
>We are culturing several different types of cells, hybridomas,
>lymphoma cells (human and mouse) and CTL lines.  We are using RPMI
>plus 10% Fetal Bovine Serum and suplimented with penicillin + 
>streptomycin.  Recently some jelly like garbage has started turning
>up in several different cell lines.  It does not overgrow the cells
>or even cause them to grow much more slowly than usual.  Some of the
>cells seem to get caught in it.  We tried filtering the media and 
>the stuff seemed to disapear, but when we put some fresh media into
>a flask (in the absence of any cells) the stuff appeared in a day or
>two and then did not change over the period of about a week.  While
>it does not appear to be doing anything harmfull, I am somewhat
>paranoid about anything strange in our cell cultures.  I don't know
>what it is (or even if it is alive), although I do know perfectly
>well what other contaminents such as yeast or bacteria look like.
>Any insights would be greatly appriciated (either by email or followup).
>                                           Thanking you in advance
>                                           Erik Wilson
>|    \   o IO             | "If we do not find     | Erik Wilson              |
>|-----|      o GANYMEDE   |anything pleasant, at   | University of Illinois   |
>|   O |    o EUROPA       |least we will find      | (217) 359-7547           |
>|----/         o CALLISTO |something new" -Voltaire| erwg0233 at uxa.cso.uiuc.edu|

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