Protectin & Singlase

[Jim Owens]

In article <9305100115.AA12036 at cscgpo.anu.edu.au> ,
Klaus.Matthaei at anu.edu.au writes among other things:
>
>Does anyone have a good method for generating single stranded probes
(sense
>and antisense) by PCR for in situs for example?
>
I don't know if asymmetric PCR generated fragments are good for in situ
hybridization, but they are quite good for sequencing with USB's
Sequenase.

Jim Owens
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Speaking only for myself; mot for my Uncle Sam.