Scanning linker deletions

Geoff Kornfeld geoffk at cumulus.csd.unsw.OZ.AU
Tue May 11 18:13:50 EST 1993




A while ago I came across a method for scanning linker deletions that involved
random nicking of the target in a plasmid followed by strand cleavage (by S1?)
then insertion of a linker, removal of the fragment from the plasmid and
isolation of modified fragment based on size.  I would like to use this
technique but cannot for the life of me remember where I saw it since the
actual paper that contained the method did not interest me at the time. PLEASE,
can someone direct me to a source for the method.  (I think that the paper was
in either Cell or Mol. Cell. Bio.)

Thanks,
Geoff Kornfeld
geoffk at cumulus.csd.unsw.oz.au



More information about the Methods mailing list