CNBr cleavage of MBP-fusion protein?

batu erman erman at brandeis.edu.bitnet
Wed May 12 12:04:13 EST 1993


In article <1993May10.162348.1 at mscf.med.upenn.edu>, kaya at mscf.med.upenn.edu
wrote:
> 
> To anyone using the pMal protein fusion and purification system from NEB or 
> anyone familiar with cyanogen bromide cleavages:
> 
> Are any of you using cyanogen bromide to cleave the fusion protein?
> 
> I have been told by somebody that the maltose (10mM)in the column elution 
> buffer can cause incomplete cleavage in the cleavage step.  It was recommended
> that I dialyze before the cleavage step.
> 
> Can anyone confirm this observation? 
> 
> I would appreciate any feedback I can get on this.  Thanks!
> 
> Angie.

I don't know if this has been discussed before, but could someone tell me
whether cyanogen bromide can cleave fusion proteins from the pGEX fusion
proteins containing the glutathiones-s-transferase protein. Can someone
post the CnBr cleaving site?
Thanks, 
Batu Erman



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