CNBr cleavage of MBP-fusion protein?
erman at brandeis.edu.bitnet
Wed May 12 12:04:13 EST 1993
In article <1993May10.162348.1 at mscf.med.upenn.edu>, kaya at mscf.med.upenn.edu
> To anyone using the pMal protein fusion and purification system from NEB or
> anyone familiar with cyanogen bromide cleavages:
> Are any of you using cyanogen bromide to cleave the fusion protein?
> I have been told by somebody that the maltose (10mM)in the column elution
> buffer can cause incomplete cleavage in the cleavage step. It was recommended
> that I dialyze before the cleavage step.
> Can anyone confirm this observation?
> I would appreciate any feedback I can get on this. Thanks!
I don't know if this has been discussed before, but could someone tell me
whether cyanogen bromide can cleave fusion proteins from the pGEX fusion
proteins containing the glutathiones-s-transferase protein. Can someone
post the CnBr cleaving site?
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