Partial/erroneous mRNA processing seen with RACE

Martin TRICK at JII.AFRC.AC.UK
Thu May 13 10:07:00 EST 1993


We have recently been using dT-magnetic beads to isolate mRNA templates for
RACE-PCR. We have been finding many products which appear to correspond to
partially processed transcripts and some are weird things which appear to
be the results of excision of cryptic introns from within exon sequences.
We suppose the relative crudity of the prep means we're isolating nuclear
RNAs, but is this a general finding from this type of methodology or is it
specific to the genes we're studying (plant receptor kinases). Does it happen
with RACE on templates made with traditional dT-cellulose columns?


Martin Trick,  Trick at uk.ac.afrc.jii 
  



More information about the Methods mailing list