NaOAc in seq gels

Michael Holloway ca566 at cleveland.Freenet.Edu
Thu May 13 19:57:34 EST 1993


In a previous article, drm21 at mbuk.bio.cam.ac.uk () says:

>
>Hi Netters,
>
>Some time ago there was a discussion on the net about using 1M NaOAc in the
>bottom buffer reservoir during sequencing in order to mimic a gradient gel.
>I have been trying this and have a problem which I wondered if anyone could
>shed light on:  In the bottom third of the gel, the sequences spread
>outwards, so that the sequences at the edges are pushed right off the gel.

Sounds like a gel casting problem to me.  The manner in which the gel is
poured and allowed to set can produce some strange effects that I wouldn't
have been able to predict.

Mike
mhollowa at ccmail.sunysb.edu

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E-mail: mhollowa at ccmail.sunysb.edu (mail to freenet is forwarded)
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