Change in Vero cell line?

Zoltan Penzes PENZES at IRAD.AFRC.AC.UK
Sun May 16 10:01:00 EST 1993


Hi,
I'am passaging IBV in Vero cells, and I noticed a change in the cell
line after about 10-15 passages. This change significantly affected
the virus growth.
Could it be that the Vero cells can only be used for such a short
period, and then have to go back to the frozen stuff? It seems to
me, that the later passages contain some elongated fibroblast like cells,
and the cell sheet looks more "crowded", and spotty.
Any ideas?
 
Zoltan Penzes
IAH, Compton, UK
 



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