(none)

pip at VENT.NEB.COM pip at VENT.NEB.COM
Mon May 17 12:03:36 EST 1993


>I want to use PCR to label a small PCR product with 32P-dATP.
>All the recipes I've seen use enormous quantities of labelled
>nucleotide. Does anyone know (or have a reference for) the
>enzyme's Km for deoxynucleotides, so I can put in the minimum
>amount of label, but still get incorporation?
>
>Thanks,
>Andy Phillips (PHILLIPSA at LARS.AFRC.AC.UK)
>Long Ashton Research Stn, Bristol, UK

Taq polymerase has a Km for dNTP's of about 16 uM, for DNA about 1.4 nM
(Kong et al., 1993, JBC 268(3)1965-75).

Paul Riggs

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