35 S contamination of PCR machine;inventory

MPKDEKKE at rulgl.LeidenUniv.nl MPKDEKKE at rulgl.LeidenUniv.nl
Tue May 18 04:29:00 EST 1993


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From: MPKDEKKE at nl.LeidenUniv.rulgl
Subject: 35 S contamination or PCR machine; inventory
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Dear Netters,

We recently discovered that after cycle sequencing with 35 S dATP in 
thin walled reaction tubes ( GeneAmp) in a PE 480 thermocycler, our
thermocycler was pretty contaminated with 35 S.

It seems that the the tubes are permeable for nucleotides because only
the lower part of the heat block was contaminated. I wonder is this
only limited to nucleotides or can small primers also " leave" your tube?

I'm interested to hear some experiences from other users. If this is a
general problem I will inform Perkin Elmer about this limitation of their
tubes.
Please drop me a mail if you encountered the same problem.

Ben Dekker
MOGEN International 
email: mpkdekke at rulgl.leidenuniv.nl



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