Storage of proteins at -20C/-70C

[Ding Ming]

>
>	Does anyone know what concentration of glycerol I should use when 
>storing protein at -20C and -70C?  Reference(s)?


My personal experience told me that 50% is enough for this purpose. 
you can dialysis your protein preparation solution in 50% glycerol 
and the water inside the bag will go out and stay on the surface of 
the outside solution. Then you can easily remove the water. You can 
repeat this process untill no water goes out and accumulates on the 
surface. Good luck!



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    Ding Ming                     
    (608)265-3544                   ming at calshp.cals.wisc.edu
    Sweet Protein Section           ming at zeus.ahabs.wisc.edu
    Taste Research Lab               
    Department of Animal Health & Biomedical Sciences    
    University of Wisconsin-Madison, 1655 Linden Dr., Madison, WI53706      
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