HA epitope tag

Dr. S. Bhattacharya sbhattac at crc.ac.uk
Wed May 19 08:20:37 EST 1993


Hi all
We are trying to immmunoprecipitate (from total cell lysate) an epitope 
tagged protein using 50mMTris7.5, 150mMNacl, 2mM MgCl2, 1mMEDTA, 0.5%NP40.
The epitope is the HA tag YPYDVPDYA with the MAb 12CA5.  Does anyone
have a better method for immunoprecipitation, and also44;1H
western/insitu with this antibody.


Many thanks
Shoumo



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