The end of the World

Chong-Yee Khoo cyk10 at cus.cam.ac.uk
Wed May 19 06:50:11 EST 1993


Don't know if this will help.

I use Promega's Magic MiniPreps (not Maxi, though we've got one on
order!),
on 2 mls of culture, and taking 18 microlitres of the final 50 microlitre
eluate
(without quantitating the DNA - sloppy!). This I subject to alkaline
denaturation,
using the method in their Protocols and Applications Guide). Gives nice
results
every time. Perhaps there's a difference between the Mini and the Maxi?

I use a modified Sequenase protocol (for microtitre plates).

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Chong-Yee Khoo                      E-mail: cyk10 at cus.cam.ac.uk
Wellcome/CRC Institute              Talk:   cyk10 at cy-mac.welc.cam.ac.uk
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