help needed for oligo screening

Mr Zhao Zhuoshen mcbzzs at nusunix1.nus.sg
Thu May 20 21:40:38 EST 1993


dear net-friend,
I am using degenerate oligo(19 mer designed according to peptide
sequence) to screen cDNA library. The Tm of my oligo is about 42 C. The
hybridization condition I am using is as following:
0.5M NaHPO3(ph6.8)
7% SDS
10% Formamide
35 C overnight.
My problem is that I can not get any signal by using this protocol.
Has anyone on the net any experience or suggestion about oligo
screening?  All respones are welcomed.
Thanks in advance.

Jackson
mcbzzs at nusunix3.nus.sg



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