help needed for oligo screening

Mr Zhao Zhuoshen mcbzzs at
Thu May 20 21:40:38 EST 1993

dear net-friend,
I am using degenerate oligo(19 mer designed according to peptide
sequence) to screen cDNA library. The Tm of my oligo is about 42 C. The
hybridization condition I am using is as following:
0.5M NaHPO3(ph6.8)
7% SDS
10% Formamide
35 C overnight.
My problem is that I can not get any signal by using this protocol.
Has anyone on the net any experience or suggestion about oligo
screening?  All respones are welcomed.
Thanks in advance.

mcbzzs at

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