help needed for oligo screening
Mr Zhao Zhuoshen
mcbzzs at nusunix1.nus.sg
Thu May 20 21:40:38 EST 1993
I am using degenerate oligo(19 mer designed according to peptide
sequence) to screen cDNA library. The Tm of my oligo is about 42 C. The
hybridization condition I am using is as following:
35 C overnight.
My problem is that I can not get any signal by using this protocol.
Has anyone on the net any experience or suggestion about oligo
screening? All respones are welcomed.
Thanks in advance.
mcbzzs at nusunix3.nus.sg
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