cycle sequencing with 35S??

ottcr at ottcr at
Tue May 25 11:34:19 EST 1993

In article <C7AB34.35M at>, jrichmn at (Joy Richman) writes:
>Dear Netters,
>Has anyone out there got cycle sequencing to work using 35 S? If so I
>would like to know what kit you used and how much dsDNA you started with.
>I would like to get away from using 32 P since we are not doing it often
>enough to get through even the smallest amount of gamma 32 P before it
>P.S. We have the Gibco kit but they do not recommend its use with 35 S.

    Have you considered 33P ? It seems to be ideally suited to 
    thermal cycle sequencing, with advantages over both 35 S
    and 32 P.
    Send me E-Mail and I could give you more specific info.
Regards, Charly Ott.

More information about the Methods mailing list