Touchdown PCR

Ethan Strauss IO00865 at MAINE.MAINE.EDU
Thu May 27 09:19:14 EST 1993


Hello,
   We are trying to do PCR on transgenic plants to amplify the transgene.
We have been getting a lot of non-specific hybridization (bands showing
up in both the controls and the transgenics). The control plasmid works
well. I was wondering if anyone has a refrence or protocol for doing
"touchdown PCR" to amplfy the specific bands of interest.
Any help is appreciated,
Ethan Strauss                              IO00865 at maine.maine.edu
Dept. Plant Biology & Pathology            208 Maple St. Apt#1
University of Maine at Orono 04469         Bangor ME, 04401
207-581-2994                               207-947-6017



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