PCR primers design
s24303 at secc.ecc.u-tokyo.ac.jp
Thu May 27 07:16:19 EST 1993
Hi! We are trying to PCR-cloning by degenerate primers. What strategy
is better to design primers deduced from AA-sequence?
1.use Inosine for (A,T,C,G)mixture? or not?
2.short primers with a smaller combinations of primers or
longer ones with a bigger combinations?
3.addition of restriction enzyme sites to both ends of primers
would be a drawback for the DNA amplifications?
If they have been already discussed, please inform me.
Thank you for cooperation.
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