Use of KCl for PAGE staining

ryan at mbcf.stjude.org ryan at mbcf.stjude.org
Fri May 28 11:10:09 EST 1993


In article <01GXUU6XH88Y000EJT at nic.the.net>, SHAUN%JASON.DECNET at relay.the.net ("Shaun D. Black") writes:
>
> There was a recent report in BioTechniques (12:564-573 (1992)) entitled 
> "Reverse Staining of Sodium Dodecy Sulfate Po;yacrylamide Gels by Imadazole
> -Zinc Salts: Sensitive Detection of Unmodified Proteins".  I haven't used 
> the method, but their data look quite good.  The stain uses readily 
> accessible reagents, has very low background, is rapid, is as sensitive as 
> a silver stain, is reversible, and can be used with Western Blotting and
> Edman sequencing.  Let us know if anyone finds this useful.  Cheers.  -Shaun
>   =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= 
>   = Shaun D. Black, PhD     | Internet:  shaun%jason.decnet at relay.the.net = 
>   = Dept. of Biochemistry   | University of Texas Health Center  at Tyler =
>   =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= 

I recently tried this imidazole/zinc procedure: although the sensitivity was
*great*, the bands were not permanently visible. Signal faded completely after 
a short time in H2O. Any hints from other users on achieving permanence?

Thanks,
Kevin.
==========================================
Kevin W. Ryan
Department of Virology & Molecular Biology
St. Jude Children's Research Hospital
Memphis, Tennessee, U.S.A.
  phone (901) 522-0411, fax (901) 523-2622
    Internet: ryan at mbcf.stjude.org
==========================================



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