pUC4KIXX sequence

MYGALP01 at ulkyvm.louisville.edu MYGALP01 at ulkyvm.louisville.edu
Mon Nov 1 22:46:30 EST 1993


In article <751931720snz at genesys.demon.co.uk>
Duncan at genesys.demon.co.uk (Duncan Clark) writes:
 
>
>The sequence for the TN903 kanamycin cassette in pUC4K was published by
>Calgene Inc. in NAR volume 16 No. 1 page 358. The Accession No. is X06404.
>
Dima,
Duncan is right about pUC4K and its derivatives pUC4-KISS and KSAC. However,
pUC4-KIXX was constructed using Km resistance gene from Tn5. Its
sequence was reported by Beck et al,1982, Gene 19, 327 (Accession No. X57709).
The fragment used in pUC4-KIXX also contains bleomycine resistance (Mazodier
et al, 1985, NAR 13, 195 (Acc. No. L19385).
Some information about all these plasmids can be found in Pharmacia '89 catalog
 
Michael Galperin, pH D.                 Bitnet: mygalp01 at ulkyvm
U of L - Microbiology                    Voice: (502) 588-7539
Louisville, KY  40292                      Fax: (502) 588-7531



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