Pichia pastoris expression system

awalley at molbiol.ox.ac.uk awalley at molbiol.ox.ac.uk
Mon Nov 1 12:19:34 EST 1993


Dear all,

Does anyone out there have any experience of the yeast expression system which 
Invitrogen has been advertising recently?

The yeast in question is Pichia pastoris and they claim that you can get your 
eukaryotic protein correctly post-translationally processed and even excreted 
into the medium. This would be ideal for expressing the enzyme I am interested 
in so does anyone have any opinions on how well this system lives up to its 
description?

Thanks in advance,

Andrew Walley,
Institute of Molecular Medicine,
Oxford, UK.



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