blocking agents for hybs

nishir at nishir at
Tue Nov 2 12:23:42 EST 1993

Hi netters,

What is the consensus out there for the efficiency (ie., ability to reduce
background) of various agents for blocking membranes that will be hybridized
with DNA or RNA probes?  (Has anyone directly compared these methods with the
same probe?)  The reagents most commonly used seem to be sheared salmon (or
herring) sperm DNA, heparin, or blotto (milk with azide).  

Rae Nishi
Portland OR 

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