Preperative PCR ?
Leonard N. Bloksberg
bloksber at pilot.msu.edu
Wed Nov 3 15:37:00 EST 1993
Anybody ever try preperative PCR? Any advice/comments?
I need 1-2 milligrams of my dsDNA 27mer oligo. I had the bugger
synthesized up before on a small scale and cloned a multimer into
pBlueScript (a 6mer, actually). I see my options as:
1. Order a preperative synthesis of more oligo ($300-).
2. Gel isolate the 6mer from 10-20mg of plasmid (will cost
from $200- to $1000- in restriction enzyme, depending on
how close to 1U/ug we can get away with).
3. Large scale preparative PCR (no cost worked up yet, due to
no protocol to base it on).
Any ideas? input? advice?
Thanks
Leonard N. Bloksberg
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