TGGE - Mutation Detection::DGGE

Basavaraju Shankarappa bsh at MED.PITT.EDU
Wed Nov 3 10:13:45 EST 1993


Dear Chris:
	The last I heard, DIAGEN company (presently Qiagen) which used to 
make TGGE apparatus called DGGE had stopped making them.  We had one in
our lab and tried couple of things.  But the results were not as 
promising as everyone thought it would be.  May be you should also 
consider other more simpler methods for this purpose.  See

Myers et al Detection of single base substitutions by RNAse cleavage at
	mismatches in DNA:RNA duplexes Science 230:1242 (1985)

Cotton et al Reactivity of cytosine and thymine in single base pair mismatches
	with hydroxylamine and osmium tetroxide and its application to
	the study of mutations.  PNAS 85:4937 (1988)

Ganguly and Prockopp Detection of single base mutations by reaction of 
	DNA heteroduplexes with a water soluble carbodiimide followed \
	by primer extension.  NAR 18: 3933 (1990)
Best,
Raj Shankarappa
bsh at med.pitt.edu
> Temperature Gradient Gel Electrophoresis (TGGE)
> ***************************************
> I am attempting to use TGGE to identify new mutations. I am interested in
> discussing this technique with any one that has an interest/experience.
> Chris Williams



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