Biotin-PCR

Barbara B Grossmann dr277 at cleveland.Freenet.Edu
Thu Nov 4 15:31:43 EST 1993


Try Biotin-14-dCTP or Biotin-14-dATP instead of the Biotin-dUTP.
Taq may not incorporate the dUTP as readily as the others.  We
use a 3:1 ratio of labeled dNTP to unlabeled dNTP. Works fine.
Please reply to DR277, Barb.  I don't have a mail box.
Michele Paris, USB



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