Problems with lamzapII in XL1Blue
Martin at gene.unp.ac.za
Thu Nov 4 13:13:04 EST 1993
I have been trying to rescue bluescript m13 from XL1 Blue lysogenised
with lambda zapII containing a +-5Kb insert. However when I do a prep of
the rescued plasmid I find the insert has somehow dissapeared - this
dissapearence sometimes corresponds with the loss of the polycloning site
but not always. I have recently managed to obtain a plasmid preparation
which appears to be about 80% Bluescript M13 with no insert and 20%
containig something else???? (apparently pBluescript with a part of the
origional insert still present but its difficult to tell). I was wondering
whether somebody had encountered the same problem and if so how they managed
to overcome it.
Thank you in advance to anybody who is able to offer advice.
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