dNTPs in PCR
THREADGILLGJ at MAIL.BECKMAN.COM
Thu Nov 4 02:43:07 EST 1993
Sorry for the last posting, I hit the wrong key
Here is the question
While trouble-shooting a PCR rxn, we accidentally
added 8 times too much dNTPs (all four). This seemed
to inhibit the rxn. Is this likely, or did we do
something else wrong?
: Patricia L. Foster
: Boston University School of Medicine
: Boston, MA USA
: pfoster at bu.edu
Patricia L. Foster
Boston University School of Medicine
Boston, MA USA
pfoster at bu.edu
We accidentally added 10 times too much dNTPs and got no amplification.
So it seems likely to me!
threadgillgj at mail.beckman.com
More information about the Methods