Concentrating small, dilute DNA
vawter at world.std.com
Thu Nov 4 21:32:54 EST 1993
In article <2999.2cc8407e at mbcl.rutgers.edu>, <swiatek at mbcl.rutgers.edu> wrote:
> Does anyone know of the best way to concentrate (i.e. ppt) small
> DNAs (<300bp)? I'm using Promega's Magic PCR preps, and the DNA is
> eluted in 40 to 50ul of buffer (or water, in my case). Currently,
> I have about 2ug DNA in 80ul H2O and need to concentrate it for
> ligation. Which salts work best? Also, is isopropanol or EtOH better?
> Thanks for any and all help...
If the DNA is in H2O, the easiest way to concentrate it would
seem to me to be to reduce the volume in a vacuum centrifuge.
vawter at harvard.edu
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