multiple PCR rxn's

Helge Weissig helgew at LJCRF.EDU
Sat Nov 6 14:21:19 EST 1993


Hi netters,

        how do you set up 40 different PCR rxn's w/o killing the enzyme or
w/o not getting any bands?

I did it the other day by premixing a rxn cocktail on ice incl enzyme. Of
course i ran out of cocktail for the last tube (even though the cocktail
was calculated for 41 rxn's) so i had to pipet everything for that rxn
anew. That last rxn came out the best, while all the other 39 rxn's were
kinda f..... (only faint or missing bands).

clueless,
helge

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