multiple PCR rxn's

Helge Weissig helgew at LJCRF.EDU
Sat Nov 6 14:21:19 EST 1993

Hi netters,

        how do you set up 40 different PCR rxn's w/o killing the enzyme or
w/o not getting any bands?

I did it the other day by premixing a rxn cocktail on ice incl enzyme. Of
course i ran out of cocktail for the last tube (even though the cocktail
was calculated for 41 rxn's) so i had to pipet everything for that rxn
anew. That last rxn came out the best, while all the other 39 rxn's were
kinda f..... (only faint or missing bands).



      ***        		Helge Weissig    	     	       	       	       	
    * . . *      		La Jolla Cancer Research Foundation
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