Creeping sequencing gels...

Martin Kennedy mkennedy at chmeds.ac.nz
Sun Nov 7 23:13:41 EST 1993


In article <9311051436.AA17623 at deimos.med.pitt.edu>, bsh at MED.PITT.EDU (Basavaraju Shankarappa) writes:

> To avoid this, I usually pre run my sequencing gels at much reduced voltage
> and then once the samples are loaded, I come up to the usual voltage.
> 
> As far as I know, the idea of prerunning the gels is to provide a royal passage
> before everybody for the gooky ion stuff to pass through the gel and not 
> inhibit our DNA.  There is no rule that pre run has to be at the same voltage
> that you use for actual run.
> 
> Raj Shankarappa

I gave up pre-running my gels about the time I stopped fixing them (4 or so
years ago) with no obvious problems.

Cheers,

Martin

NNNN   NN  Martin A Kennedy (E-mail = mkennedy at chmeds.ac.nz)  ZZZZZZZ  
NN NN  NN       Cytogenetic and Molecular Oncology Unit          ZZZ
NN  NN NN           Christchurch School of Medicine            ZZZ
NN   NNNN              Christchurch, New Zealand              ZZZZZZZ
	      Phone (64-3)364-0880  Fax (64-3)364-0750



More information about the Methods mailing list