Creeping sequencing gels...

Martin Kennedy mkennedy at
Sun Nov 7 23:13:41 EST 1993

In article <9311051436.AA17623 at>, bsh at MED.PITT.EDU (Basavaraju Shankarappa) writes:

> To avoid this, I usually pre run my sequencing gels at much reduced voltage
> and then once the samples are loaded, I come up to the usual voltage.
> As far as I know, the idea of prerunning the gels is to provide a royal passage
> before everybody for the gooky ion stuff to pass through the gel and not 
> inhibit our DNA.  There is no rule that pre run has to be at the same voltage
> that you use for actual run.
> Raj Shankarappa

I gave up pre-running my gels about the time I stopped fixing them (4 or so
years ago) with no obvious problems.



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