Ribonucleotide analogs

delihas at ASTERIX.BIO.SUNYSB.EDU delihas at ASTERIX.BIO.SUNYSB.EDU
Mon Nov 8 09:22:34 EST 1993


	Does anyone know of a ribonucleotide analog that can be incorporated
  by T7 RNA polymerase and which will render the resulting RNA resistant to
  RNases?  I am trying to make an affinity column for an RNA binding protein
  but a pesky nuclease activity keeps co-purifying with the protein of
  interest.  I tried Rnasin and Inhibitace with little success. Any ideas?

					Craig DeLoughery
					delihas at asterix.bio.sunysb.edu




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