Ribonucleotide analogs
delihas at ASTERIX.BIO.SUNYSB.EDU
delihas at ASTERIX.BIO.SUNYSB.EDU
Mon Nov 8 09:22:34 EST 1993
Does anyone know of a ribonucleotide analog that can be incorporated
by T7 RNA polymerase and which will render the resulting RNA resistant to
RNases? I am trying to make an affinity column for an RNA binding protein
but a pesky nuclease activity keeps co-purifying with the protein of
interest. I tried Rnasin and Inhibitace with little success. Any ideas?
Craig DeLoughery
delihas at asterix.bio.sunysb.edu
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