Protein electrophoresis: pH of sample buffer

Michael Holland M.Holland at
Tue Nov 9 20:37:48 EST 1993

This is the sort of embarrasing question that when you are asked by a
graduate student you feel you should know the answer but don't:
Why is the ph of the sample buffer in Laemmli systems 6.8 when the stacking
gel it is entering is 8.9?? Can it be altered to be alkaline?
Michael K Holland PhD
CSIRO, Wildlife & Ecology
PO Box 84, Lyneham ACT 2602, Australia
Fax: 61-6-242-9242  Phone:61-6-242-1793
M.Holland at

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