Cloning Pfu|

John Cooper cooperj at
Tue Nov 9 12:23:30 EST 1993

In article <1993Nov3.110034.19155 at> GENETICA%IPCUCSC.earn at ("L.Frigerio") writes:
>Path: nih-csl!lhc!biosci!daresbury!daresbury!news
>From: GENETICA%IPCUCSC.earn at ("L.Frigerio")
>Newsgroups: bionet.molbio.methds-reagnts
>Subject: Cloning Pfu|
>Message-ID: <1993Nov3.110034.19155 at>
>Date: 3 Nov 93 11:44:58 GMT
>Sender: list-admin at
>Distribution: bionet
>Lines: 12
>Precedence: first-class
>Original-To: "." <methods at>
>Hi net,
>does anybody have experience with cloning of Pfu-amplified sequences?
>We tried to use standard blunt-end cloning and Klenow filling of the fragments
>but nothing seems to work...Any suggestions or informations will be greatly app
>reciated. Thanks in advance.
>Lorenzo Frigerio
>Istituto di Genetica Vegetale
>UCSC Piacenza-Italy
>genetica at ipcucsc.bitnet
>botanica at

Try the strategene pCR-SK cloning kit. I use it for cloning these fragments.
Recombination is about 70% for white colonies-- good enough

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