Are PCR contions different from RAPD?
huangbx at DEAKIN.EDU.AU
Thu Nov 11 16:54:31 EST 1993
I optimized my PCR reaction conditions with a specific primer set. Once
everything has been finalized, I changed to RAPD with random primers from
UBC (yes, the annealling temperature lowered to 35oC). Upto now I have
scaned 30 random primers. There are NO PRIMERS shown any good RAPD
Therefore I have questions:
Are PCR reaction conditions different from those of RAPD? If so, how can I
optimize my RAPD reaction conditions? Well, I will be very appreciated if
someone kindly email his RAPD protocol to me.
Thank you very much for your time.
huangbx at deakin.edu.au
FAX:61 52 272022.
School of Biological and Chemical Sciences, Deakin University, Geelong
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