Are PCR contions different from RAPD?

Huang Bixing huangbx at DEAKIN.EDU.AU
Thu Nov 11 16:54:31 EST 1993


Hello netters,

I optimized my PCR reaction conditions with a specific primer set. Once 
everything has been finalized, I changed to RAPD with random primers from 
UBC (yes, the annealling temperature lowered to 35oC). Upto now I have 
scaned 30 random primers. There are NO PRIMERS shown any good RAPD 
results yet.

Therefore I have  questions:  
Are PCR reaction conditions different from those of RAPD? If so, how can I 
optimize my RAPD reaction conditions? Well, I will be very appreciated if 
someone kindly email his RAPD protocol to me.

Thank you very much for your time.

With regards.

Bixing Huang
huangbx at deakin.edu.au
FAX:61 52 272022.
-----

School of Biological and Chemical Sciences, Deakin University, Geelong
-----



More information about the Methods mailing list