Protein electrophoresis: pH of sample buffer

Jonathan B. Marder MARDER at
Wed Nov 10 20:18:03 EST 1993

In article <41870.M.Holland at> M.Holland at ("Michael Holland") writes:
>Subject: Protein electrophoresis: pH of sample buffer
>From: M.Holland at ("Michael Holland")
>Date: 10 Nov 93 01:37:48 GMT

>This is the sort of embarrasing question that when you are asked by a
>graduate student you feel you should know the answer but don't:
>Why is the ph of the sample buffer in Laemmli systems 6.8 when the stacking
>gel it is entering is 8.9?? Can it be altered to be alkaline?
The pH of the stacking gel is 6.8!  The resolving gel is pH 8.9.  The
combination of pH and ionic strength is what causes stacking.
Jonathan B. Marder                 '
Department of Agricultural Botany  |     Internet: MARDER at AGRI.HUJI.AC.IL
The Hebrew University of Jerusalem | /\/ Bitnet:   MARDER at HUJIAGRI
Faculty of Agriculture             |/  \ Phone:    (08 or +9728) 481918
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