Protein electrophoresis: pH of sample buffer

Jonathan B. Marder MARDER at agri.huji.ac.il
Wed Nov 10 20:18:03 EST 1993


In article <41870.M.Holland at dwe.csiro.au> M.Holland at dwe.csiro.au ("Michael Holland") writes:
>Subject: Protein electrophoresis: pH of sample buffer
>From: M.Holland at dwe.csiro.au ("Michael Holland")
>Date: 10 Nov 93 01:37:48 GMT

>Hi
>This is the sort of embarrasing question that when you are asked by a
>graduate student you feel you should know the answer but don't:
>Why is the ph of the sample buffer in Laemmli systems 6.8 when the stacking
>gel it is entering is 8.9?? Can it be altered to be alkaline?
                       ****
The pH of the stacking gel is 6.8!  The resolving gel is pH 8.9.  The
combination of pH and ionic strength is what causes stacking.
__
Jonathan B. Marder                 '
Department of Agricultural Botany  |     Internet: MARDER at AGRI.HUJI.AC.IL
The Hebrew University of Jerusalem | /\/ Bitnet:   MARDER at HUJIAGRI
Faculty of Agriculture             |/  \ Phone:    (08 or +9728) 481918
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