E. coli genomic preps

Patricia Foster pfoster at bu.edu
Thu Nov 11 13:01:49 EST 1993


Dear Netters,
     What with all the discussion of DNA purification
kits, I thought I would ask if anyone knows of a quick
prep for E. coli genomic DNA.  We have tried the CTAB
protocol in Current Protocols with little success.
I want to both clone and PCR for sequencing.  So far
I am unhappy with results.  Any hints would be appreciated.
Thanks
pat
--
Patricia L. Foster
Boston University School of Medicine
Boston, MA USA
pfoster at bu.edu



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