belas at mbimail.umd.edu
Fri Nov 12 15:17:14 EST 1993
I have a couple of questions regarding preparation of ssDNA from pBLUESCRIPT
II using the M13K07 helper phage. First, can I propagate the helper phage
using the same protocols I use for regular M13 (adding kan for selection).
Second, would someone point me to a good technique for using phagemids? I
currently have Sambrook et al and Ausabel et al in front of me. Are there any
other sources for methodology that I should look at? Thanks.
~~ "Now imagine a Moebius vortex inside a spherical constant, and you've ~~
~~ got my cosmology." Frank Zappa, 1992. ~~
~~ Bob Belas, Assistant Professor, Sensory Transduction ~~
~~ Center of Marine Biotechnology, University of Maryland ~~
~~ Biotechnology Institute, 600 East Lombard Street ~~
~~ Baltimore (my fair city) MD 21202 ~~
~~ (410) 783-4825 Belas at mbimail.umd.edu ~~
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