M13K07/Phagemid help

Bob Belas belas at mbimail.umd.edu
Fri Nov 12 15:17:14 EST 1993


I have a couple of questions regarding preparation of ssDNA from pBLUESCRIPT 
II using the M13K07 helper phage.  First, can I propagate the helper phage 
using the same protocols I use for regular M13 (adding kan for selection).  
Second, would someone point me to a good technique for using phagemids?  I 
currently have Sambrook et al and Ausabel et al in front of me.  Are there any 
other sources for methodology that I should look at?  Thanks.



~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
~~ "Now imagine a Moebius vortex inside a spherical constant, and you've ~~
~~               got my cosmology."  Frank Zappa, 1992.                  ~~
~~                                                                       ~~
~~         Bob Belas, Assistant Professor, Sensory Transduction          ~~
~~        Center of Marine Biotechnology, University of Maryland         ~~
~~           Biotechnology Institute, 600 East Lombard Street            ~~
~~                Baltimore (my fair city) MD  21202                     ~~
~~         (410) 783-4825                 Belas at mbimail.umd.edu          ~~
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~



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