Protocol for SDS Removal from proteins
mbrgw at s-crim1.dl.ac.uk
Mon Nov 15 12:24:32 EST 1993
In article <1993Nov15.155434.4465 at emr1.emr.ca> weathere at emr1.emr.ca (Carl Weatherell) writes:
>I'm trying to recover some proteins from prep-scale chromatographic
>fractions containing SDS. Can someone point me to or supply me with a
>facile route to recover these proteins without the SDS?
Chlorofrom/methanol extraction, according to
Wessel and Fluegge (1984) Analytical Biochemistry 138, 141-143.
I use it all the time, and it works a treat.
Robin Walters. Robert Hill Institute, Sheffield UK.
A fact is an opinion that everyone agrees with.
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