Protocol for SDS Removal from proteins

R.G. Walters mbrgw at s-crim1.dl.ac.uk
Mon Nov 15 12:24:32 EST 1993


In article <1993Nov15.155434.4465 at emr1.emr.ca> weathere at emr1.emr.ca (Carl Weatherell) writes:
>I'm trying to recover some proteins from prep-scale chromatographic
>fractions containing SDS.  Can someone point me to or supply me with a
>facile route to recover these proteins without the SDS?

Chlorofrom/methanol extraction, according to

Wessel and Fluegge (1984) Analytical Biochemistry 138, 141-143.

I use it all the time, and it works a treat.

--
Robin Walters.                      Robert Hill Institute, Sheffield UK.

A fact is an opinion that everyone agrees with.



More information about the Methods mailing list