denomme at FHS.CSU.MCMASTER.CA
Mon Nov 15 12:40:32 EST 1993
On 11 Nov 1993, Fourie Joubert wrote:
> I'm interested in the computer modelling of antibody-antigen binding.
> Especially, in the possibilities of mutagenesis to enhance specificity and/
> or affinity. Thus, I would like to do my predictions on computer, and then
> try them out in the lab by recombinant means.
> Can anybody direct me towards references concerning similar work?
> Please mail, any help would be enormously appreciated!
> _/_/_/_/ _/_/_/_/_/ Fourie Joubert
> _/ _/ Department of Biochemistry
> _/ _/ University of Pretoria
> _/_/_/_/ _/ South Africa
> _/ _/ fourie at ccnet.up.ac.za
> _/ _/_/_/_/ Disclaimer: "Fourie who?"
I know of two papers that have used molecular modeling to study Ig V
Tomiyama Y, Brojer E, Ruggeri ZM et al. A molecular model of RGD ligands.
Antibody D gene segments that direct specificity for the integrin
alphaIIb-beta3. JBC 1992;267:18085-18092.
Hughes-Jones NC, Bye JM, Beale D and Coadwell J. Nucleotide sequences and
three-dimensional modelling of the VH and VL domains of two human
monoclonal antibodies specific for the D antigen of the human
Rh-blood-group system. Biochem J 1990;268:135-140.
Hope this helps,
denomme at FHS.McMaster.ca
More information about the Methods