PCRing an intron (problems)

Martin Leach leach at mbcrr.harvard.edu
Tue Nov 16 04:05:09 EST 1993


In article <2c8tki$hfd at news.bu.edu>, reichert at bu.bu.edu (Tanya Reichert)
wrote:

> For the past month, I've been trying work out a technique for detecting
> copy number of a certain gene from genomic DNA of varied animal species
> via PCR.  The idea is to take flanking exon sequences that are very well
> conserved across species, construct degenerate primers from them, and 
> amplify the intron that they delimit.  Assuming that the intron size varies
> from gene to gene within a species, the number of PCR products should 
> reflect the copy number of the gene.

It is known that within the same gene across different species that the
size of the intron differs in size. This could account for why you do not
see it in some species, as it is too large to PCR.

-- 

.....          Martin Leach                Email:leach at mbcrr.harvard.edu 
   _|____      Dept. of Pharmacology       Phone: (617) 638-5323        
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