LB vs. TB?

Jim Owens jow at helix.nih.gov
Tue Nov 16 09:51:17 EST 1993


In article <2c8tn6INNcn6 at emory.mathcs.emory.edu> T. J. Murphy,
medtjm at bimcore.emory.edu writes:
>My guess is the big factor is the glycerol in TB as carbon source is 
>what makes the T in TB.

In article <753390924snz at genesys.demon.co.uk> Duncan Clark,
Duncan at genesys.demon.co.uk writes:
>TB is certainly better in that one gets I a culture with a much higher
OD. I

For what it's worth, several years ago when I was still doing "maxi
preps" I followed OD with time.  On simultaneous preps of the same
plasmid from the same bacterial clone, the OD remained linear up to 0.5
in LB, but up to 1.2 in TB.

Good luck,

Jim Owens



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