DNA from decalcified tissue?

Ola Myklebost olam at radium.uio.no
Tue Nov 16 12:36:36 EST 1993

I really need DNA from some old tumor specimens (paraffin blocks) which
were treated with 20% HCl to remove bone (osteosarcoma). We have good
experience in purifying DNA from paraffin blocks, but HCL???? Will it all
be depurinated? Or irreversibly precipitated together with all other stuff?

Ola Myklebost             Email  ola.myklebost at dnr.uio.no
Dept of Tumor Biology
Inst for Cancer Research
The Norwegian Radium Hospital
N-0310 OSLO, Norway
Tel +47-22-50-60-50, xtn 9830, Fax +47-22-52-24-21

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