DNA from decalcified tissue?

Ola Myklebost olam at radium.uio.no
Tue Nov 16 12:36:36 EST 1993


I really need DNA from some old tumor specimens (paraffin blocks) which
were treated with 20% HCl to remove bone (osteosarcoma). We have good
experience in purifying DNA from paraffin blocks, but HCL???? Will it all
be depurinated? Or irreversibly precipitated together with all other stuff?

-- 
Ola Myklebost             Email  ola.myklebost at dnr.uio.no
Dept of Tumor Biology
Inst for Cancer Research
The Norwegian Radium Hospital
N-0310 OSLO, Norway
Tel +47-22-50-60-50, xtn 9830, Fax +47-22-52-24-21



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