RNA extraction

Michael Man mman at molbio.cbs.umn.edu
Tue Nov 16 11:54:31 EST 1993


I am going to isolate RNA from small amout of tissue (less than 0.1gm). I have
experience dealing with larger scale isolation from 1 gm of tissue.  I need you
suggestion on how to deal smaller scale isolation.  I need your DETAILed 
recommemdation, such as size of homogenizer, type of centrifuge tube, ....
Anyway the detailed , the better.  I use the Guanidium Thiocyanate/phenol method.
Thanks.

michael
mman at molbio.cbs.umn.edu 
"A person without plan is a fly without a head." 



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