Hoefer TK-100 fluorimeter/SS-DNA

BLC GBGA13 at VMS3.GLASGOW.AC.UK
Wed Nov 17 12:15:10 EST 1993


Path: NewsWatcher!user
From: gbga13 at uk.ac.gla.vms (Bernard Cohen)
Newsgroups: bionet.molbio.methds-reagnts
Followup-To: bionet.molbio.methds-reagnts
Subject: HOEFER TK-100 AND SS-DNA
Message-ID: <gbga13-171193180710 at 130.209.132.71>
Organization: University of Glasgow

I am about to have a week's trial of a Hoefer TK-100 fluorimeter.   A major interest is in the quantitation of SS-DNA sequencing template, for which the bis-benzimide fluor is of limited (=no?) use.

Can the system be tweaked to assay SS-DNA by adding an excess of, say, random hexamers?   The idea being that they would bind to the SS-DNA forming stoichiometric amounts of DS which would fluoresce.   The obvious complication is that the hexamers would form DS with themselves too, but this could be treated as a reagent blank.

Can anyone with enough expertise in molecular-association kinetcs comment on this idea?

Is there any other method in existence?

-- 
Bernie Cohen             
Genetics Dept
University of Glasgow
Glasgow G11 5JS           Tel: +44 (41) 339-8855 ext 5103
Scotland. UK.             Fax: +44 (41) 330-5994



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