LB or not LB, that is the question

Andrew Healey andrew at qimr.edu.au
Wed Nov 17 01:25:38 EST 1993


Various posts so far:

>: There seems to be 2 recipies for "LB" in the literature.
>: My recollection is that it stood for Luria-Bertani Broth and 
>: contained 5g NaCl per litre.
>: Modern recipies have 10g NaCl per litre. 
>
>Advanced Bacterial Genetics (1980) Davis, Botstein, Roth 5g/l
>Sambrook et al (1989) 10g/l
>
>To further confuse the issue, I found an "L broth (complex medium for
>rapid growth of _E. coli_ and other _Enterobacteriaceae_) in Manual of
>Methods for General Bacteriology (1981), p238.
>
>NaCl ... 0.5 g (yes 1/2 gram)

Hmmm...the earliest Maniatis(1982) also has 10g/L.
When I started out in molecular biology, a lot of people referred
to LB as L-Broth (logical) but I was given to understand, as stated
above, that Luria-Bertani medium is correct. Anyway BIO101 makes both,
with LB at 10g/L NaCl, and L-broth at 0.5g/L. Maybe somewhere the 
decimal point got left out? giving VLB (virtual LB) at 5g/L.
BTW, who is Bertani?



~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
email: andrew at qimr.edu.au                              *
Tel:   07-362-0376                               *
Fax:   07-362-0105                                         *
                                                       *
The Bancroft Centre
Queensland Institute of Medical Research            *
300 Herston Road, Brisbane, AUSTRALIA 4029

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~



More information about the Methods mailing list