Direct sequencing of PCR products

Donna Jensen JOFTOPSM at VTVM1.CC.VT.EDU
Wed Nov 17 13:34:54 EST 1993


Hi everyone.  I am interested in directly sequencing a PCR product that
is approximately 300 bp, and I was wondering if anyone could point me to
references or send me a method for doing so.  The organism I work with is
about 70% GC, so single-stranded sequencing is preferable to double-stranded.

Thanks in advance.

Donna Jensen
JOFTOPSM at VTVM1.CC.EDU



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